SAP97/A0015

Synaptic-associated protein 97 (SAP97 or A0015) is a scaffold protein that belongs to the membrane-associated guanylate kinase (MAGUK) family (PSD93, PSD95, SAP97, SAP102).

Synaptic-associated protein 97 (SAP97 or A0015) is a scaffold protein that belongs to the membrane-associated guanylate kinase (MAGUK) family (PSD93, PSD95, SAP97, SAP102). SAP97 is expressed in different tissues during embryo development and widely expressed in adult mice. SAP97 is localized in the basolateral membrane of polarized cells. In the brain, it is mainly expressed in the cerebellum, cerebral cortex, hippocampus and olfactory bulb. Alternative splicing produces three different isoforms. The predicted 905 amino acid sequence contains three PDZ domains, a SH3 domain, a guanylate kinase-homology domain and a L27 domain that interact with receptors, potassium channels, associated signaling proteins and cytoskeletal proteins. Over-expression of SAP97 in embryonic primary neurons increases the amount of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors on the surface, spine enlargement and an increase in miniature excitatory post-synaptic current (mEPSC). SAP97 knock-out mice have craniofacial abnormalities, small kidneys, shorter ureters, reduced nephron formation and die a few hours after birth.